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A375 cell lysates from human malignant melanoma cells were cultured in the media containing 10% FBS.
Background
Cells were lysed in lysis buffer (50mM Tris(PH7.4), 150mM NaCl, 1% NP-40, 1% SDS). 100 ¦Ìg of cellular protein was mixed with 4 times volume of 5X SDS-PAGE loading buffer.
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