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其他中文名称 ： V8蛋白酶S
英文名称 ： Protease S Aureus
其他英文名称 ： Endoproteinase Glu-C from Staphylococcus aureus V8；V8 Protease
C A S ： 66676-43-5
级 别 ： BR
来 源 ： Staph aureus V8
Activity ： ≥500units/mg
活力定义 ： One Unit causes a change of 0.001 A280 nm per minute at 37℃, pH 7.8 using casein as the substrate
产品描述 ： Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976).
性 状 ： 粉末。
用 途 ： 生化研究。
保 存 ： 2～8℃
