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Ó¢ÎÄÃû³Æ£º 6¡ÁHIS Antigen
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Synthetic peptide: HHHHHH
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Full name |
HHHHHH Tag |
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Synonyms |
6 His epitope tag |
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Background |
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
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