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021-54845833/15800441009
克隆类型： mouse monoclonal
技术规格
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Background: |
The blue fluorescent protein (BFP) is a mutant of the green fluorescent protein, where the phenolic ring of the chromophore has been replaced by imidazole cycle of histidine residue. The usability of BFP as a fluorescent marker is hampered by its low fluorescence quantum yield at room temperature. The intensity of fluorescence increases by a factor of 4.5 when the temperature is decreased from 320 K down to 225 K. The fluorescence is also enhanced by hydrostatic pressure. Both effects have been explained by shift of the equilibrium between hydrogen nonbonded and hydrogen-bonded chromophores. |
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Applications: |
WB |
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Name of antibody: |
mBFP |
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Immunogen: |
This antibody is raised against a recombinant protein and recognizes mBFP. |
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Full name: |
monomeric blue fluorescence protein |
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SwissProt: |
P80893 |
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WB Predicted band size: |
26 kDa |
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WB Positive control: |
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mBFP ne) cDNA for 48 hrs and lysed |
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WB Recommended dilution: |
1000-5000 |
