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中文名称 ： 荧光素酶(萤火虫)
其他中文名称 ： 荧光素酶；重组型酵素
英文名称 ： Luciferase firefly
其他英文名称 ： Luciferase from Photinus pyralis (firefly)
C A S ： 61970-00-1
级 别 ： BR
product line ： BioUltra
Assay ： ≥98% (SDS-PAGE)
Mol wt ： mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)
Protein ： 10-35% E1%/280
Measuring range ： ≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)
Foreign activity ： ATPase ≤5 nanomolar units/mg protein；Nucleoside diphosphokinase ≤20 nanomolar units/mg protein
活力定义 ： One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C
产品描述 ： Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.
Note : Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units
性 状 ： 粉末。EC：1.13.12.7
用 途 ： 生化研究。
保 存 ： -20℃。保质期1年
