ÆäËûÖÐÎÄÃû³Æ £º Á×Ëá¶þõ¥Ã¸¢ò£»Á×Ëá¶þõ¥Ã¸
Ó¢ÎÄÃû³Æ £º Phosphodiesterase II from bovine spleen
ÆäËûÓ¢ÎÄÃû³Æ £º 3¡ä-Exonuclease£»Spleen exonuclease£»Spleen phosphodiesterase
C A S £º 9068-54-6
¼¶ ±ð £º BR
Activity £º ¡Ý10u/mg
»îÁ¦¶¨Òå £º One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37¡æ, pH 6.5, with an RNA substrate
²úÆ·ÃèÊö £º Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique
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021-54845833/15800441009
