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Background: |
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
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Applications: |
WB, IP, IF, IHC |
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Name of antibody: |
PCNA |
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Immunogen: |
Fusion protein of human PCNA |
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Full name: |
proliferating cell nuclear antigen |
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Synonyms: |
ATLD2 |
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SwissProt: |
P12004 |
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IHC positive control: |
Human colorectal carcinoma |
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IHC Recommend dilution: |
200-400 |
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WB Predicted band size: |
29 kDa |
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WB Positive control: |
CHO-K1, C6, COS7, 3T3 and Hela cell lysates |
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WB Recommended dilution: |
1000-5000 |
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IF positive control: |
Hela cells |
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IF Recommend dilution: |
50-100 |
